AAV Manufacturing Process Development Using PATfix HPLC Analytics

Time: 4:15 pm
day: Day One


  • When the expression of the AAV results in less than 10% full capsid it is quite impossible to reach better than 90% full capsid in the final product. It is therefore mandatory to optimise the expression to result in better empty/full ratio
  • This can be realised by using at-line HPLC to allow for analysis of the full and empty capsids ratio in the bioreactor filtrate from the transfection to the harvest
  • The residual empty capsids can be removed by polishing purification step using different anion exchange and multimode columns